USF HEALTH NORTH
- USF
- Office of Clinical Research
- BSL-3 Laboratory
- Human Functional Perfomance Laboratory
- Motion Analysis Laboratory
- Biostatistics Core
- Moffitt Core Facilities
- VA Hospital Resources
- Hyperbaric Chamber
USF HEALTH SOUTH
- TGH Office of Clinical Research
- TGH Core Facilities
- Biomechanical Testing Laboratory
- Osman Microsurgery Laboratory
- Biostatistics Core
USF HEALTH
ST. PETERSBURG
ST. PETERSBURG
- Flow Cytometry
- Tissue depository
- Genomics
- Vevo770
USF MAIN
CAMPUS RESOURCES
CAMPUS RESOURCES
Digital Leica DM4000 uright microscope
This instrument supports standard fluorescent imaging and is equipped with fluorescent filter sets for DAPI, FITC, GFP and TRITC, and two higly sensitive CCD cameras. The LAS (Leica Application Suite) program provides you with all features necessary to control the microscope and camera for acquiring excellent images. In addition, it has a Prior motorized stage and focus drives for image analysis interfaced with the Stereologer System supporting computerized quantitative analysis of biological tissues including; cell counts, volume, surface area and length.
DM4000 B microscope
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Lenses:
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2.5x/0.07 PLAN
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5x/0.12 PLAN FLUOTAR
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10x/0.3 PLAN FLUOTAR
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20x/0.5 PLAN FLUOTRA
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40x/1.25-0.75 PLAN APO Oil
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63x/1.4-0.60 PLAN APO Oil
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Fluorescent filters: A4 (UV filter for DAPI and Hoechst); N2.1 (G/R filter for TRITC, propidium iodide, and DsRed); I3 (B/G filter for FITC, Alexa fluor 488); GFP (for GFP)
CCD camera
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Leica DFC350 FX camera
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1.4 Mpixel CCD with very high sensitivity in th visible and infrared spectrum.
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12 bit monochrome
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Up to 60 images/second for imaing fast bleaching fluorescence samples.
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Toshiba CCD
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Applications This microscope equipped with monochrome and color cameras provides excellent fluorescence and color images. Color camera is very useful for acquiring real color images of histologically stained sample. Monochrome camera with digital pseudocoloring provides multicolor image of fluorescently labeled sample. Indeed, with samples that are about less than 10 mm in thickness, the quality of images is quite comparable to those obtained by confocal microscopy.
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